EGFR-AS1 Promotes Bladder Cancer Progression by Upregulating EGFR

Figure 5

EGFR-AS1 promotes EGFR expression by maintaining its RNA stability. (a) The EGFR-AS1 and EGFR sequences have partial complementarity in the genome. (b) The correlation analysis between the EGFR-AS1 expression and EGFR expression in the TCGA database. (c) Knocking down EGFR-AS1 resulted in lower expression of EGFR mRNA in bladder cancer cells. RNA stability were measured using Actinomycin D. Knocking down EGFR-AS1 decreased the stability of EGFR mRNA in 5637 (d) and T24 cell (e), maximize at 4 hours. Overexpressing EGFR-AS1 enhanced the stability of EGFR mRNA in 5637 (f) and T24 cell (g).

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