Decellularized Aortic Scaffold Alleviates H₂O₂-Induced Inflammation and Apoptosis in CD34+ Progenitor Cells While Driving Neovasculogenesis

Figure 4

Decellularized aortic scaffold inhibited H₂O₂-induced apoptosis in vitro. Apoptosis was detected by FACS in CD34+ progenitor cells treated with 50 μmol/L or 100 μmol/L H₂O₂ in the absence or presence of decellularized aortic scaffold. (a) CD34+ cells only. (b) CD34+ cells exposed to 50 μmol/L H₂O₂. (c) CD34+ cells exposed to 100 μmol/L H₂O₂. (d) CD34+ cells in the presence of a decellularized aortic scaffold. (e) CD34+ cells exposed to 50 μmol/L H₂O₂ in the presence of a decellularized aortic scaffold. (f) CD34+ cells exposed to 100 μmol/L H₂O₂ in the presence of a decellularized aortic scaffold. (g) Apoptotic ratios (percentages of apoptotic CD34+ cells) were calculated in both groups when treated with no H₂O₂, 50 μmol/L H₂O₂, or 100 μmol/L H₂O₂. Compared with CD34 group, , , , respectively.

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