Synergistic Utilization of Necrostatin-1 and Z-VAD-FMK Efficiently Promotes the Survival of Compression-Induced Nucleus Pulposus Cells via Alleviating Mitochondrial Dysfunction

<div>Nec-1 (20 <i>μ</i>M) or 3-MA (5 mM) attenuated 24 and 36 h compression-induced NP cells autophagy. (a, b) Western blot and quantitative analysis of autophagy-related molecules LC3B-II, Beclin1, and GAPDH in NP cells. (c, d) Representative dot plot images by flow cytometry after MDC staining and quantitative analysis of MDC positive ratio in NP cells. Data from treated groups have been normalized to GAPDH. 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BioMed Research International

fig1

Figure 1

Figure 1: Synergistic Utilization of Necrostatin-1 and Z-VAD-FMK Efficiently Promotes the Survival of Compression-Induced Nucleus Pulposus Cells via Alleviating Mitochondrial Dysfunction 

Figure 1 | Synergistic Utilization of Necrostatin-1 and Z-VAD-FMK Efficiently Promotes the Survival of Compression-Induced Nucleus Pulposus Cells via Alleviating Mitochondrial Dysfunction 