Synergistic Utilization of Necrostatin-1 and Z-VAD-FMK Efficiently Promotes the Survival of Compression-Induced Nucleus Pulposus Cells via Alleviating Mitochondrial Dysfunction

<div>3-MA (5 mM) had little effect on NP cells necroptosis, and Nec-1+3-MA achieved almost the same effect as Nec-1 treatment in preventing compression-induced NP cells death. (a, b) Western blot and quantitative analysis of necroptosis-related molecules RIPK1, pRIPK1, RIPK3, pRIPK3, MLKL, and GAPDH in NP cells. (c) RT-PCR measured the mRNA expression of necroptosis-related genes RIPK1, RIPK3, MLKL, and GAPDH in NP cells. (d) CCK-8 assay showed the viability change of NP cells. (e) LDH release exhibited the cytotoxicity of NP cells. 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BioMed Research International

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Figure 2

Figure 2: Synergistic Utilization of Necrostatin-1 and Z-VAD-FMK Efficiently Promotes the Survival of Compression-Induced Nucleus Pulposus Cells via Alleviating Mitochondrial Dysfunction 

Figure 2 | Synergistic Utilization of Necrostatin-1 and Z-VAD-FMK Efficiently Promotes the Survival of Compression-Induced Nucleus Pulposus Cells via Alleviating Mitochondrial Dysfunction 