Synergistic Utilization of Necrostatin-1 and Z-VAD-FMK Efficiently Promotes the Survival of Compression-Induced Nucleus Pulposus Cells via Alleviating Mitochondrial Dysfunction

<div>Nec-1 (20 <i>μ</i>M) or Z-VAD (20 mM) attenuated 24 and 36 h compression-induced NP cells apoptosis. (a, b) Western blot and quantitative analysis of apoptosis-related molecules Cleaved Caspase-3, Cleaved Caspase-8, and Cleaved Caspase-9 and GAPDH in NP cells. (c, d) Representative dot plot images of apoptosis NP cells by flow cytometry after Annexin V staining and quantitative analysis. Data from treated groups have been normalized to GAPDH. 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BioMed Research International

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Figure 3

Figure 3: Synergistic Utilization of Necrostatin-1 and Z-VAD-FMK Efficiently Promotes the Survival of Compression-Induced Nucleus Pulposus Cells via Alleviating Mitochondrial Dysfunction 

Figure 3 | Synergistic Utilization of Necrostatin-1 and Z-VAD-FMK Efficiently Promotes the Survival of Compression-Induced Nucleus Pulposus Cells via Alleviating Mitochondrial Dysfunction 