Synergistic Utilization of Necrostatin-1 and Z-VAD-FMK Efficiently Promotes the Survival of Compression-Induced Nucleus Pulposus Cells via Alleviating Mitochondrial Dysfunction

<div>Z-VAD (20 mM) enhanced 24 and 36 h compression-induced NP cells necroptosis. (a, b) Western blot and quantitative analysis of necroptosis-related molecules RIPK1, pRIPK1, RIPK3, pRIPK3, MLKL, and GAPDH in NP cells. (c) RT-PCR measured the mRNA expression of necroptosis-related genes RIPK1, RIPK3, MLKL, and GAPDH in NP cells. 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BioMed Research International

fig4

Figure 4

Figure 4: Synergistic Utilization of Necrostatin-1 and Z-VAD-FMK Efficiently Promotes the Survival of Compression-Induced Nucleus Pulposus Cells via Alleviating Mitochondrial Dysfunction 

Figure 4 | Synergistic Utilization of Necrostatin-1 and Z-VAD-FMK Efficiently Promotes the Survival of Compression-Induced Nucleus Pulposus Cells via Alleviating Mitochondrial Dysfunction 