S100A9 Increases IL-6 and RANKL Expressions through MAPKs and STAT3 Signaling Pathways in Osteocyte-Like Cells

Figure 2

The effect of S100A8 and S100A9 on mRNA expressions of proinflammatory cytokines in osteocytes. MLO-Y4-A2 cells were cultured in α-MEM containing S100A8 (50 nM) or S100A9 (50 nM) for 24 hours, and mRNA expressions of IL-1β, IL-6, IL-8, andTNF-α were analyzed by RT-PCR.