S100A9 Increases IL-6 and RANKL Expressions through MAPKs and STAT3 Signaling Pathways in Osteocyte-Like Cells

Figure 3

Dose effects of S100A8 and S100A9 on IL-6 and RANKL expressions in osteocytes. MLO-Y4-A2 cells were treated with S100A8 (10–50 nM), S100A9 (10–50 nM), and Pg LPS (500 ng/ml) for 24–48 hours. ((a) and (c)) After 24 hours of stimulation, IL-6 and RANKL mRNA expressions were analyzed by qRT-PCR. ((b) and (d)) IL-6 and RANKL production levels were measured using ELISA after treatment with a reagent for 48 hours. Data are means ± SD (n = 6). and show significant differences between the indicated groups.

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