Ephrin-A5 Is Involved in Retinal Neovascularization in a Mouse Model of Oxygen-Induced Retinopathy

Figure 1

The expression patterns and distribution of Efna5 in OIR mice. (a) Retinas of OIR mice and C57BL/6J mice were harvested at P12, P13, P15, and P17, and the Efna5 mRNA level was measured with RT-PCR. The RT-PCR result was a representative of three independent experiments. (b) The relative expression level of Efna5 was calculated by normalizing the optical density of the Efna5 band to the GAPDH band. (c) Retinas of OIR mice and C57BL/6J mice were harvested at P12, P15, and P17. Immunofluorescence staining of Efna5 was performed with an anti-Efna5 antibody (red). Immunofluorescence staining of the endothelial cell was performed with Isolectin-B₄ (green). Scale bars represent 50 μm; P: postnatal day; +: OIR mouse; -: C57BL/6J mouse; ILM: internal limiting membrane; GCL: ganglion cell layer; IPL: inner plexiform layer; INL: inner nuclear layer; OPL: outer plexiform layer; ONL: outer nuclear layer; OS: outer segment.

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