Mir-141-3p Regulates Apoptosis and Mitochondrial Membrane Potential via Targeting Sirtuin1 in a 1-Methyl-4-Phenylpyridinium in vitro Model of Parkinson’s Disease

Figure 3

miR-141-3p regulated apoptosis, ROS production, and mitochondrial membrane potential in the PD model. (a) Differentiated PC12 cells were treated with MPP+ (800 μM, 24 h). RT-qPCR showed that MPP+ treatment increased the miR-141-3p expression significantly. (b) RT-qPCR confirmed the transfection efficiency of the miR-141-3p mimic and inhibitor. (c) RT-qPCR results showed that the miR-141-3p expression increased significantly after miR-141-3p mimic transfection compared with the control group, while the miR-141-3p expression decreased significantly after the miR-141-3p inhibitor transfection. (d) CellROX Deep Red staining showed that miR-141-3p mimic transfection increased ROS production, while miR-141-3p inhibitor transfection decreased ROS production. (e) Annexin V/PI staining showed that miR-141-3p mimic increased the apoptosis percentage while the miR-141-3p inhibitor decreased the apoptosis percentage. . Error bars indicate standard deviation (SD).

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