Mir-141-3p Regulates Apoptosis and Mitochondrial Membrane Potential via Targeting Sirtuin1 in a 1-Methyl-4-Phenylpyridinium in vitro Model of Parkinson’s Disease

Figure 4

miR-141-3p negatively regulated SIRT1 by direct binding to its 3-UTR. (a) The predicted binding sites of miR-141-3p and SIRT1. (b) The miR-141-3p mimic and inhibitor was transfected into PC12 cells. Western blot showed that miR-141-3p mimic decreased the SIRT1 protein. The miR-141-3p inhibitor increased the SIRT1 protein. (c) RT-qPCR showed that miR-141-3p mimic decreased SIRT1 mRNA. The miR-141-3p inhibitor increased SIRT1 mRNA. (d) SIRT1 3-UTR-wild-type or SIRT1 3-UTR-mutant reporter was transfected along with miR-141-3p mimic into PC12 cells. The luciferase activity was decreased significantly when miR-141-3p mimic was cotransfected with SIRT1 3-UTR-wild-type reporter. . Error bars indicate standard deviation (SD).

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