Mir-141-3p Regulates Apoptosis and Mitochondrial Membrane Potential via Targeting Sirtuin1 in a 1-Methyl-4-Phenylpyridinium in vitro Model of Parkinson’s Disease

Figure 5

SIRT1 regulated the related protein expression. (a) Western blot results showed that the SIRT1 overexpression downregulated Nos1, p-Iκb, TNF-α, IL-1β, and IL-6 and upregulated the Bcl-2 protein expression. SIRT1 siRNA upregulated Nos1, p-IκB, TNF-α, IL-1β, and IL-6 and downregulated Bcl-2 levels in MPP+-treated PC12 cells. (b) The effect of SIRT1 on the secretion of inflammatory factors was determined by ELISA. TNF-α, IL-1β, and IL-6 levels decreased after the SIRT1 overexpression. SIRT1 depletion upregulated TNF-α, IL-1β, and IL-6 levels. . Error bars indicate standard deviation (SD).

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