Mir-141-3p Regulates Apoptosis and Mitochondrial Membrane Potential via Targeting Sirtuin1 in a 1-Methyl-4-Phenylpyridinium in vitro Model of Parkinson’s Disease

Figure 7

miR-141-3p regulated Nos1 and Δψm through SIRT1 in PC12 cells. (a) Transfection of miR-141-3p mimic decreased Δψm, and SIRT1 activator resveratrol increased Δψm in MPP+-treated PC12 cells. The miR-141-3p inhibitor increased Δψm, and the SIRT1 inhibitor sirtinol decreased Δψm. (b) Western blot showed that miR-141-3p mimic upregulated the Nos1 level, which was downregulated by resveratrol treatment. The miR-141-3p inhibitor downregulated the Nos1 protein level, which was slightly upregulated by sirtinol treatment. . Error bars indicate standard deviation (SD).

(a)

(b)