Inhibition of Distinct Proline- or N-Acetylglucosamine-Induced Hyphal Formation Pathways by Proline Analogs in Candida albicans

Figure 5

Effect of proline analogs. (a) Effect of AZC on different concentration of FBS yeast-to-hyphal transition. (b) Effect of simultaneous addition of T4C and mPro on yeast-to-hyphal transition in different concentration of FBS. C. albicans (10⁵ cells/mL) with various compounds (final concentration was 20 mM) were incubated at 37°C for 4 h. At least 30 cells were counted and all assays were triplicated. Standard deviations are shown for each sample. (c) Effect of proline analogs on yeast form growth of C. albicans. C. albicans cells (10⁵ cells/mL) in Saboraoud’s medium were cultured with 20 mM AZC, 20 mM T4C, and 20 mM mPro, or without at 30°C and OD 660 nm was recorded every 15 minutes by biophotorecorder.

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