Disease Mutation Study Identifies Critical Residues for Phosphatidylserine Flippase ATP11A

Figure 5

PS flippase activity assay of ATP11A mutants. (a–g) NBD-PS labeling assays revealed a reduced amount of PS transported into the cells for ATP11A Y300F and D913K mutants. Flow cytometry histograms of NBD-PS labeling assays were shown in a–f. The -axis represented the NBD-PS fluorescence intensity of intact living cells. The -axis showed numbers of NBD-PS-labeled cells. PcDNA3.1-C-3xflag empty plasmid was transfected as a negative control (NC) (a). PS flippase activity measured by the mean fluorescence intensity of ATP11A mutant transfected cells (f). ATP11A-WT was used as control and its activity was set as 1.0. . . The data represented .

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