Treatment with Minocycline Suppresses Microglia Activation and Reverses Neural Stem Cells Loss after Simulated Microgravity

Figure 1

Experimental procedure and time course of tail suspension, BrdU administration, and animal perfusion. The rats in the tail suspension (TS) groups received tail suspension for 7, 14, or 28 d (indicated by green lines in (1), (2), and (3), respectively) and were sacrificed for proliferation assays. Twenty-four hours before killing (indicated by the arrow in (1), (2), and (3), respectively), these rats received three pulses of BrdU every 8 h for 24 h (indicated by yellow lines in (1), (2), and (3), respectively). The rats used for differentiation assays first received BrdU (100 mg/kg) injection each day for 7 d (indicated by yellow line in (4)) and then were perfused at day 21 (indicated by the arrow in (4)) after treatment with control or tail suspension.