CDKN2A Polymorphism in Melanoma Patients in Colombian Population: A Case-Control Study

Table 1

Primer pairs used in the conventional PCR reaction and HRM-qPCR reaction.

(a)

Conventional PCR
Exon	Primers 5-3	Product size (pb)	Melting temp. (°C)	Nucleotide sequence 5-3
1	F	399	61.0	CTGCAAACTTCGTCCTCCAGA
	R			CCCTTGCCTGGAAAGATACC
2	F	479	61.0	GGTGAGGGGGCTCTACACAA
	R			GGTCTCCCGGGCTGAACTTT

(b)

HRM–qPCR assay
RS	SNP	Protein change	Primers 5-3	Product size (pb)	Melting temp. (°C)	Nucleotide sequence 5-3
rs104894097	c.71G>C	p.R24P	F	79	63.3	CCGCCTCCAGCAGCG
			R			ATGGAGCCTTCGGCTGACT
rs104894095	c.159G>C	p.M53I	F	121	59.0	GGGCTCTACACAAGCTTCCTT
			R			GTGGAGCAGCAGCAGCT
rs104894094	c.301G>T	p.G101W	F	99	61.0	GAGGGCTTCCTGGACACG
			R			CTCCTCAGCCAGGTCCAC
rs104894098	c.377T>A	p.V126D	F	88	61.0	CGTGGACCTGGCTGAGGA
			R			GGCATGGTTACTGCCTCTGG
rs3731249	c.442G>A	p.A148T	F	100	61.0	GGGCACCAGAGGCAGTAAC
			R			GCTTTGGAAGCTCTCAGGGTA