β-Sitosterol Alleviates Inflammatory Response via Inhibiting the Activation of ERK/p38 and NF-κB Pathways in LPS-Exposed BV2 Cells

Figure 5

The effect of BS on the activation of the MAPK pathway. The cells were pretreated with BS for 2 h prior to the stimulation of LPS (100 ng/mL). After 2 h, the cell pellet was collected and extracted the total protein. After that, the expression levels of p-ERK, ERK (a, b), p-JNK, JNK (a, c), p-p38, p38(a, d), and β-actin were detected by western blot. The results were presented as (). ^## versus the control group. and versus the LPS-stimulated group.

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