Fluvastatin-Pretreated Donor Cells Attenuated Murine aGVHD by Balancing Effector T Cell Distribution and Function under the Regulation of KLF2

Figure 5

The expression of surface molecules related to T cell migration on T cells in transplanted recipients. BALB/c mice were transplanted with bone marrow plus T cells from Fluvastatin or buffer pretreated C57BL/6 donors. Single-cell suspension of the thymus, spleen, lymph nodes, and blood were acquired from each recipient on days 3, 7, 14, 21, and day 28 posttransplantation and subsequently gated on CD3⁺ T cells by flow cytometry to analyze (a) CD62L, (b) CCR7, (c) S1P1, (d) CD44, and (e) CXCR3 expression. Data represents the of results from 3 independent experiments. There were 4-5 mice in each time point in Fluvastatin and buffer GVHD groups, respectively, and 4 mice were sacrificed in unmanipulated group. , , n.s . (f) One representative FACS data of the spleen on day 14 from 3 independent experiments was shown, and the number in the corner means the population percentage.