Research Article
Fluvastatin-Pretreated Donor Cells Attenuated Murine aGVHD by Balancing Effector T Cell Distribution and Function under the Regulation of KLF2
Figure 6
Cytokines production after Fluvastatin treatment. (a) Splenic mononuclear cells from C57BL/6 mice treated with Fluvastatin or buffer for consecutive 7 days were harvested. Cells were cultured with PMA (50 ng/μl), ionomycin (1 μg/μl) for 3-5 hours, and brefeldin A (5 μg/μl) was added before flow cytometric analysis of TNF-α and IFN-γ production in CD3+ T cells. One representative FACS data from 3 independent experiments was shown in the right panel, and the number in the corner represents the percentages of gated cells. (b) BALB/c mice were used as recipients following transplantation of Fluvastatin- or buffer-pretreated C57BL/6-derived bone marrow and splenic cells. Splenic cells from recipients were stimulated as described above, and then, the production of TNF-α and IFN-γ in CD8+ and CD4+ T cells on days 7, 14, 21, and 28 were detected. Data represents the of results from 3 experiments ( per time point). , , n.s . (c) Representative FACS plots on day 21 were shown. The number in the corner means the cell percentages.
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