Study of the Relationship between Microbiome and Colorectal Cancer Susceptibility Using 16SrRNA Sequencing

Table 2

PCR amplification reaction system (20 μL).

The reagents	Volume
5 × PCR buffer	4 μl
2.5 mM dNTP	2 μl
Template NDA	10 ng
5 μM primer	0.8 μl
FastPfu polymerase	0.4 μl
Distilled water	Up to 20 μl