Novel WT1 Target Genes: IL-2, IL-2RB, and IL-2RG Discovered during WT1 Silencing Using Lentiviral-Based RNAi in Myeloid Leukemia Cells

Figure 1

WT1-siRNA lentiviral transduction suppressed endogenous WT1 mRNA expression level at 48 hours posttransduction. RT-PCR analysis of WT1 was performed. Two specific PCR bands representing WT1(+KTS) and WT1(-KTS) transcripts were downregulated (a). WT1 protein was also reduced by WT1-siRNA lentiviral transduction. Intriguingly, no detectable full-length WT protein is observed at 72 and 96 hours posttransduction in WT1 silenced cells.

(a)

(b)