Novel WT1 Target Genes: IL-2, IL-2RB, and IL-2RG Discovered during WT1 Silencing Using Lentiviral-Based RNAi in Myeloid Leukemia Cells

Figure 3

WT1 silencing (WT1-siRNA) induced apoptosis via activation of caspase-3/7 enzyme activity of K562 cell (K562-WT1-siRNA-GFP⁺ cells) at the specific time points compared with the C-siRNA-GFP⁺ control cells (C-siRNA). Analysis of caspase-3/7 enzyme activities was performed by using the Apo-One Homogeneous Caspase-3/7 apoptosis kit (Promega).