Novel WT1 Target Genes: IL-2, IL-2RB, and IL-2RG Discovered during WT1 Silencing Using Lentiviral-Based RNAi in Myeloid Leukemia Cells

Figure 5

WT1 silencing (WT1-siRNA) downregulated the expression of IL-2, IL-2RB, and IL-2RG compared with the control cells (C-siRNA). RT-PCR analysis of IL-2, IL-2RB, and IL-2RG mRNA was performed on RNA isolated from K562-WT1-siRNA-GFP⁺ or K562 cells after WT1-siRNA lentiviral vector transduction (WT1-siRNA) and K562-C-siRNA-GFP⁺ control cells (C-siRNA). GAPDH mRNA detection was used as an internal control.