Novel WT1 Target Genes: IL-2, IL-2RB, and IL-2RG Discovered during WT1 Silencing Using Lentiviral-Based RNAi in Myeloid Leukemia Cells

Figure 6

Effect of WT1 silencing on cell cycle progression of K562 cells. Determination of cell cycle distribution of K562-WT1-siRNA-GFP⁺ compared with K562-C-siRNA-GFP⁺ control cells at 24 and 72 hours posttransduction by flow cytometry. The distribution of cells in the G0/G1, S, G2/M, and death cells was calculated and labeled. The percentage of each phase was plotted, C-siRNA population (Panel A) and WT1-siRNA-treated cell population (Panel B). The assay was performed in triplicate. Statistical significance was determined using Student’s -test; .

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