Novel WT1 Target Genes: IL-2, IL-2RB, and IL-2RG Discovered during WT1 Silencing Using Lentiviral-Based RNAi in Myeloid Leukemia Cells

Table 1

The primer sequences and PRC conditions used in RT-PCR analysis.

Genes	Primer sequences	Annealing temperature (°C)	Product size (bp)	References
WT1	R: 5-TCAAAGCGCCAGCTGGAGTTT-3; F: 5-AGACATACAGGTGTGAAACC-3	51	225 (+17AA); 215 (-17AA)	[36]
IL2	R: 5-TGGGAAGCACTTAATTATCAAGTC-3; F: 5-CGTAATAGTTCTGGAACTAAAGGG-3	60	150	[37]
IL-2RB	R: 5-CGGTGTTCCTGCAGTTG-3; F: 5-CAGTATGAGTTTCAGGTGCG-3	50	205	[37]
IL-2RG	R: 5-CCAACAGAGATAACCACGG-3; F: 5-CGCTACACGTTTCGTGTTC-3	60	152	[37]
GAPDH	R: 5GTACTCAGCGGCCAGCATCG-3; F: 5-AGCCACATCGCTCAGACACC-3	60	310	[38]