HAND2-AS1 Works as a ceRNA of miR-3118 to Suppress Proliferation and Migration in Breast Cancer by Upregulating PHLPP2

Figure 1

Overexpression of HAND2-AS1 repressed proliferation, migration, and invasion in BC cell. (a) RT-qPCR assays were constructed to evaluate expression of HAND2-AS1 in BC cell lines (MCF-7, MDA-MB-231, SK-BR-3, and MDA-MB-453) and normal mammary epithelial cell (MCF-10A). (b) RT-qPCR assays were performed to appraise the expression of HAND2-AS1 in cells transfected with pcDNA3.1/HAND2-AS1. (c, d) CCK8 and colony formation assays were conducted to appraise cell proliferation in cells transfected with pcDNA3.1/HAND2-AS1. (e) Flow cytometry analysis was performed to assess the apoptosis rate. (f, g) Transwell assays were carried out to assess the migration and invasion capacity. (h) Western blot was used to measure E-cadherin, N-cadherin, MMP2, Vimentin, and slug proteins.

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