Lipopolysaccharide Inhibits Alpha Epithelial Sodium Channel Expression via MiR-124-5p in Alveolar Type 2  Epithelial Cells

Figure 3

MiR-124-5p downregulates α-ENaC expression by binding to the 3′-untranslated region (3′-UTR). (a) The prediction of the miR-124-5p binding site located in α-ENaC mRNA. Alignment of miR-124-5p with wild-type (WT) and mutant (MT) α-ENaC mRNA 3′-UTR. (b) Dual luciferase assay for AT2 cells cotransfected with miR-124-5p negative control (NC) or miR-124-5p mimic (Mimic) together with pmirGLO-α-ENaC (WT or MT) for 24 h , compared with NC group; N = 3.

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