Hyperbranched Cationic Glycogen Derivative-Mediated IκBα Gene Silencing Regulates the Uveoscleral Outflow Pathway in Rats

Figure 1

The distribution of DMAPA-Glyp/Cy3-siRNA complexes in rat ciliary muscle after transfection via three different delivery routes (×200) ( per group). Notes. (a) Intravitreal injection group. (b) Ciliary muscle injection group—the white arrows indicate scattered fluorescence (red) in the ciliary muscle. (c–f) Intracameral injection group: (c) nuclei; (d) ciliary muscle fibers—α-SMA-positive immunofluorescence staining (green); (e) DMAPA-Glyp/Cy3-siRNA complexes (red); (f) merging of (c), (d), and (e)—the red arrow indicates DMAPA-Glyp/Cy3-siRNA complexes in the ciliary muscle. Abbreviations: cb—ciliary body; cm—ciliary muscle; ret—retina; scle—sclera; tm—trabecular meshwork; DMAPA-Glyp—3-(dimethylamino)-1-propylamine-conjugated glycogen; α-SMA—α-smooth muscle actin.

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