Hyperbranched Cationic Glycogen Derivative-Mediated IκBα Gene Silencing Regulates the Uveoscleral Outflow Pathway in Rats

Figure 5

Examination of NF-κBp65 mRNA and protein levels at 24, 48, and 72 h after RNAi. The error bars represent the standard deviation calculated from three parallel experiments ( per group). Notes. (a) NF-κBp65 mRNA expression was quantified by real-time PCR. Expression levels were normalized with GAPDH. (b) NF-κBp65 protein expression was examined by western blot. The NF-κBp65 and β-actin bands were analyzed by densitometry, and the values were normalized with β-actin, which were represented in the bar graph. Abbreviations: PBS—phosphate-buffered saline; DMAPA-Glyp—3-(dimethylamino)-1-propylamine-conjugated glycogen; NC—nonspecific control; Lipo—Lipofectamine™ 2000.

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