Hyperbranched Cationic Glycogen Derivative-Mediated IκBα Gene Silencing Regulates the Uveoscleral Outflow Pathway in Rats

Figure 6

NF-κBp65 nuclear translocation at 24, 48, and 72 h after RNAi by immunofluorescence (×400) ( per group). Notes. (a) The white arrows indicate increased nuclear expression of NF-κBp65 in the DMAPA-Glyp+siRNA group; the asterisks indicate the weak nuclear signal of NF-κBp65 in the DMAPA-Glyp+NC group; the solid line rectangle indicates the magnifying region of the nuclear expression of NF-κBp65. (b) The fluorescence level counting of NF-κBp65 in the nuclei of the ciliary muscle was quantified to confirm NF-κBp65 nuclear translocation, and the values were represented in the bar graph. The error bars represent the standard deviation calculated from three parallel experiments. , compared with the DMAPA-Glyp+NC group. Abbreviations: DAPI—4,6-diamidino-2-phenylindole; cb—ciliary body; cm—ciliary muscle; scle—sclera; DMAPA-Glyp—3-(dimethylamino)-1-propylamine-conjugated glycogen; NC—nonspecific control.

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