Hyperbranched Cationic Glycogen Derivative-Mediated IκBα Gene Silencing Regulates the Uveoscleral Outflow Pathway in Rats

Figure 7

MMP-2 mRNA expression and activity at 24, 48, and 72 h after RNAi. Notes. (a) MMP-2 mRNA expression was quantified by real-time PCR. Expression levels were normalized with GAPDH. The error bars represent the standard deviation calculated from three parallel experiments ( per group). (b) The activity of MMP-2 was analyzed by in situ gelatin zymography. The stronger the green fluorescence in the ciliary muscle, the higher the MMP-2 activity (×200). The yellow solid line indicates the area of the ciliary muscle scanned for MMP-2 activity. (c) Fluorescence level counting in the area of the ciliary muscle was performed to measure the MMP-2 activity, and the values were represented in the bar graph. The error bars represent the standard deviation calculated from three parallel experiments ( per group). and , compared with the PBS group, the DMAPA-Glyp group, and the DMAPA-Glyp+NC group. Abbreviations: PBS—phosphate-buffered saline; DMAPA-Glyp—3-(dimethylamino)-1-propylamine-conjugated glycogen; NC—nonspecific control; Lipo—Lipofectamine™ 2000; cb—ciliary body; cm—ciliary muscle; scle—sclera.

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