Sequence Analysis of the K13-Propeller Gene in Artemisinin Challenging Plasmodium falciparum Isolates from Malaria Endemic Areas of Odisha, India: A Molecular Surveillance Study

Table 2

PCR thermo cycling conditions for primary and secondary PCR (for PF3D7_1343700).

Primer name	PCR	Sequence 5-3	Product	Initial denaturation	Denaturation	Annealing	Extension	Final extension	No. of cycles
K13_PCR-F	1^0 PCR	CGGAGTGACCAAATCTGGGA	2097 bp	95-15 m	95-00.30 s	58-2.00 m	72-2.00 m	72-10.00 m	25
K13_PCR_R	1^0 PCR	GGGAATCTGGTGGTAACAGC
K13_N1_F	2^0 PCR	GCCAAGCTGCCATTCATTTG	849 bp			60-1.00 m	72-2.00 m		28
K13_N1_R	2^0 PCR	GCCTTGTTGAAAGAAGCAGA