Sema4D Aggravated LPS-Induced Injury via Activation of the MAPK Signaling Pathway in ATDC5 Chondrocytes

Figure 2

Silence of Sema4D suppressed LPS-induced cell damage in ATDC5 cells. (a, b) The expression of Sema4D mRNA and protein in ATDC5 cells was detected after transfection with siRNAs. ATDC5 cells were transfected with Sema4D siRNA1, Sema4D siRNA2, or siRNA negative control (siRNA-NC); after incubation for 48 h, LPS (5 μg/mL) was added and cultured for 12 h. and versus the control group; ^# versus the LPS+siRNA-NC treatment group. (c, d) Cell viability and apoptosis of ATDC5 cells were tested. (e) The content of IL-1β, IL-6, and TNF-α in culture medium of ATDC5 cells was measured through ELISA kits. (f) Expression of pro-caspase 1, cleaved-caspase 3, Bax, and Bcl-2 was detected through Western blotting. ATDC5 cells were transfected with Sema4D siRNA1 and treated with LPS. and versus the control group; ^# versus the LPS+siRNA-NC treatment group.

(a)

(b)

(c)

(d)

(e)

(f)