Sema4D Aggravated LPS-Induced Injury via Activation of the MAPK Signaling Pathway in ATDC5 Chondrocytes

Figure 4

Inhibition of the MAPK signaling pathway abolished the effect of Sema4D on ATDC5 cells. (a, b) The expression of p-P38, P38, Erk1/2, and p-Erk1/2 in ATDC5 cells was examined through Western blotting. (c) The expression of Sema4D in ATDC5 cells was examined after treatment with SB203580 and U1026. (d–f) Cell viability, apoptosis, and levels of IL-1β, IL-6, and TNF-α were detected. After transfection with ov-Sema4D for 48 h, LPS, SB203580, and U1026 were added and cultured for 12 h. and versus the control group, ^# versus the LPS treatment group, and ^& versus the LPS+ov-Sema4D treatment group.

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