Sema4D Aggravated LPS-Induced Injury via Activation of the MAPK Signaling Pathway in ATDC5 Chondrocytes

Figure 5

Sema4D mediated cell viability and apoptosis through interacting with its receptor Plexin B1. (a) Relative mRNA levels of Plexin B1, Plexin B2, and CD72 in ATDC5 cells were examined after stimulation with LPS for 12 h. and versus 0 μg/mL of LPS treatment group; ^# versus 5 μg/mL of LPS treatment group. (b) Expression of Plexin B1, Plexin B2, and CD72 mRNA was tested after transfection with siRNAs. versus the si-NC transfected group. (c, d) Cell viability and apoptosis were detected after transfection with si-Plexin B1, si-Plexin B2, or si-CD72 in Sema4D-overexpressed LPS-stimulated ATDC5 cells. and versus the untreated group, ^# versus the LPS+ov-Sema4D treatment group; ns versus no significant difference.

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