Pharmacological Properties of a Traditional Korean Formula Bojungchiseup-tang on 3T3-L1 Preadipocytes and High-Fat Diet-Induced Obesity Mouse Model

Figure 1

Effect of BJT on adipogenesis of 3T3-L1 cells. Preadipocytes were treated with various concentrations of BJT for 48 h, and their viability was estimated using MTT assay (a). 3T3-L1 cells were stimulated by differentiation medium in the presence or absence of indicated BJT concentrations for 8 days and subjected to ORO staining (b). The stained cells were visualized using a microscope at 100x magnification. Oil Red O was dissolved by isopropanol and measured at 510 nm (c). 3T3-L1 cells were cultured in a differentiation medium in the presence or absence of the indicated concentrations of BJT until day 6. Western blot was performed to determine the protein level of PPARγ, C/EBPα, and SRBP1. For clarity, cropped gel images are shown (d). qRT-PCR was performed to determine the mRNA level of PPARγ (e), C/EBPα (f), and SRBP1 (g). The values are represented as . ^### vs. the nondifferentiation group; , , and vs. the differentiation group; significances were determined using one-way ANOVA followed by a Dunnett’s post hoc test.

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