Hypermethylation of EFEMP1 in the Hippocampus May Be Related to the Deficit in Spatial Memory of Rat Neonates Triggered by Repeated Administration of Propofol

Figure 3

Schematic diagram of long interspersed nucleotide EFEMP1. EFEMP1 transcript consists of a 5 untranslated region (5-UTR) with internal promoter activity, an open reading frame, and a 3-UTR. The sequence shown represented a 112-base pair fragment in the 5-UTR of EFEMP1. PCR primers were designed based on the reverse complementary strands of this fragment. Numbers 1–8 refer to locations of the CpG site within the EFEMP1 elements tested, and the highlighted CpG units represented more than one CpG site detected at the same time.