[Retracted] Revealing PAK2’s Function in the Cell Division through MKLP1’s Interactome

Figure 1

The tail domain of MKLP1 is crucial for its localization and function during cell division. (a) Schematic diagram showing full-length MKLP1 and its mutants. (b, c) F-MKLP1 concentrates in the nucleus when expressed in HEK293 cells. ΔC16-MKLP1 and MSD-MKLP1 showed a higher degree of binding with microtubules and accumulated at the leading edge of the cells compared with the control cells. The arrowheads show accumulated eGFP. Scale bar, 25 μm. (d) HEK293 cells were transfected with plasmid DNA encoding TD-MKLP1 or STD-MKLP1 as indicated. Forty-eight hours after transfection, the cells were fixed, and eGFP and DAPI were detected to allow quantification of the number of mutant-expressing cells, which exhibited a bi-/multinuclear phenotype. (e) The results represent the mean (±S.E.M.) from 3 independent experiments. The overexpression of the MKLP1 tail domain resulted in a significant increase in the numbers of binuclear cells ().

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