[Retracted] Revealing PAK2’s Function in the Cell Division through MKLP1’s Interactome

Figure 3

PAK2 interact with MKLP1. (a) Synchronised HEK293 cells were fixed and stained for PAK2.The image shows a cell at anaphase and cytokinesis. PAK2 is localized to the spindle midzone (anaphase) and to the midbody (cytokinesis) at the center of the furrow, similar to the localization of MKLP1. (b) Immunoprecipitation (IP) shows endogenous MKLP1 interacting with PAK2 in HEK293 cells. Tubulin was used to normalize the inputs. IB, immunoblot. (c) Transient expression of the CFP and YFP fusion proteins in HEK293 cells, and images are shown by the CFP (A), YFP (B), and FRET (C) channels. FRET^C is calculated as described in Materials and Methods and is represented as a pseudocolor. A.l.u.f.i.: arbitrary linear units of fluorescence intensity. Bar, 20 μm. (d) FR values were obtained from individual HEK293 cells expressing the indicated fusion proteins. Data are shown as the and originate from three independent experiments. versus HEK293 cells cotransfected with CFP and YFP (negative control). Student’s -test.

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