[Retracted] Revealing PAK2’s Function in the Cell Division through MKLP1’s Interactome

Figure 4

Knockdown of PAK2 in HEK293 cells causes cytokinesis failure and bi-/multinucleation. (a) HEK293 cells were transfected with PAK2 shRNA, PAK2 shRNA plus PAK2 rescue plasmid, or scrambled shRNA as indicated. Forty-eight hours after transfection, the cells were fixed, and eGFP and DAPI were detected to allow the quantification of eGFP-expressing cells, which exhibited a bi-/multinuclear phenotype, Scale bars, 30 μm. (b) The results represent the mean (±S.E.M.) from 3 independent experiments. The knockdown of PAK2 resulted in a significant increase in the numbers of bi-/multinucleated cells (). (c) HEK 293 cells grown on coverslips were transfected with pEGFP empty plasmid, scrambled shRNA, PAK2 shRNA, or PAK2 shRNA plus PAK2 rescue plasmid. At 48 h after transfection, the cells were fixed and stained with MKLP1 antibodies (red) and DAPI (blue). Scale bars, 10 μm. (d) HEK 293 cells were treated for 2 days with PAK2 shRNA, and microscopy revealed that the living cells attempted cytokinesis but failed. Scale bars, 30 μm.

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