Redirecting Metabolic Flux towards the Mevalonate Pathway for Enhanced β-Carotene Production in M. circinelloides CBS 277.49

Table 2

Design matrix of medium optimization for β-carotene production in CBS 277.49.

Std. order	Variables			Results
	C/N ratios	Cerulenin (μg/mL)	Ketoconazole (mg/L)	Observed values	Predicted values
				β-Carotene (mg/L)	β-Carotene (mg/L)
1	10	20	100	2.93	3.21
2	40	20	100	2.24	2.73
3	10	50	100	1.09	1.24
4	40	50	100	1.91	2.02
5	10	20	200	1.96	2.33
6	40	20	200	1.65	1.98
7	10	50	200	1.93	1.93
8	40	50	200	2.84	2.84
9	0	35	150	1.81	1.45
10	50	35	150	2.14	1.81
11	25	10	150	4.26	3.80
12	25	60.3	150	2.27	2.36
13	25	35	66	2.80	2.31
14	25	35	235	2.45	2.26
15	25	35	150	2.24	1.64
16	25	35	150	1.49	1.64
17	25	35	150	1.51	1.64
18	25	35	150	1.49	1.64
19	25	35	150	1.49	1.64
20	25	35	150	1.50	1.64