Identification and Analysis of Estrogen Receptor α Promoting Tamoxifen Resistance-Related lncRNAs

Figure 1

Proposed work flow of the procedure to identify the ERα promoting tamoxifen resistance-related lncRNAs. In breast cancer tamoxifen-sensitive cell MCF-7/WT (WT) and tamoxifen-resistant cell MCF-7/TamR (TAMR), (a) estrogen receptor α (ERα) binding or no binding events are determined by ChIP-seq and the differential ERα redistribution is visualized by UCSC genome browser; (b) the differential gene expression profile is investigated by RNA-seq and presented in a heatmap. (c) By analyzing the ChIP-seq and RNA-seq data from GSE86538, the overlap genes (candidate genes) may be ERα promoted and tamoxifen resistance related. And further, these genes will be verified by functional enrichment analysis by clusterProfiler package and survival analysis via the TANRIC website.

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