Hydrogen-Rich Saline Inhibits Lipopolysaccharide-Induced Acute Lung Injury and Endothelial Dysfunction by Regulating Autophagy through mTOR/TFEB Signaling Pathway

Figure 6

mTOR activator reversed HRS protection effect and mTOR Inhibitor enhanced HRS protection effect in LPS-induced injury. (a) Western blot assay was performed to detect the expression levels of p-mTOR, p-S6 LC3-II, P62, and Beclin-1 protein expression levels. (b) Immunohistochemical assay was performed to detect the expression of TFEB protein (Scale bar = 50 μm). (c) ELISA assay was used to detect inflammatory factor levels. (d) Flow cytometry was used to detect the survival rate of HPMECs. (e) Flow cytometry was used to detect the apoptosis rate of HPMECs. All experiments of cells were repeated three times. Data were expressed as mean ± SD. Statistically significant differences: indicates . LPS: lipopolysaccharide; HRS: Hydrogen-rich saline; MHY1485: an mTOR activator; OSI-027: an mTOR inhibitor. All data were analyzed by one‐way analysis of variance (ANOVA) with Tukey’s multiple comparison post hoc test.

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