Citron Rho-Interacting Serine/Threonine Kinase Promotes HIF1a-CypA Signaling and Growth of Human Pancreatic Adenocarcinoma

Figure 2

CIT promotes the growth of PDAC cells. (a) CIT knockdown in Capan-1 and BxPC-3 cells. Capan-1 and BxPC-3 cells were infected with lentivirus carrying sh-Ctrl or sh-CIT for 24 hours, and then qPCR and western blot were applied to analyze the expression of CIT. vs. sh-Ctrl. (b) CIT knockdown reduces the rate of proliferation of PDAC cells. Capan-1 and BxPC-3 cells with stable CIT knockdown were used for cell proliferation experiments. , vs. sh-Ctrl. (c) CIT knockdown reduces the ability of colony formation in PDAC cells. Capan-1 and BxPC-3 cells with stable CIT knockdown were used for colony formation experiment, the colonies were cultured for 14 days before final analysis. vs. sh-Ctrl. (d) CIT overexpression in Capan-1 cells. Capan-1 cells were infected with lentivirus overexpressing CIT (CIT-OE) and control construct for 24 hours, then qPCR and western blot were applied to analyze the expression of CIT. vs. Ctrl. (e) CIT overexpression promotes the rate of proliferation of PDAC cells. Capan-1 cells with stable CIT overexpression (CIT-OE) were applied to cell proliferation experiments. , vs. Ctrl. (f) CIT overexpression enhances the capacity of colony formation of PDAC cells. Capan-1 cells with stable CIT overexpression were used for colony formation experiment, the colonies were cultured for 14 days before final analysis. vs. Ctrl.

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