Isoliquiritigenin Suppressed Esophageal Squamous Carcinoma Growth by Blocking EGFR Activation and Inducing Cell Cycle Arrest

Figure 2

Isoliquiritigenin (ILQ) suppressed EGFR activation in ESCC cells. A ILQ inhibited EGFR signaling pathway. ESCC cells were treated with different ILQ for 2 h, and the expression of the indicated protein was detected by western blotting. B-C, ILQ blocked EGF-induced EGFR activation dose B and time C dependently. After starvation overnight, ESCC cells were incubated with ILQ for 2 h and then stimulated with 50 ng/ml EGF, the cell lysates were collected, and the expression of indicated protein was analyzed by western blotting. D–F, EGFR knockdown in ESCC cells impaired the sensitivity to ILQ, the expression of EGFR was silenced with shRNA(D) and then treated with ILQ.

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