Isoliquiritigenin Suppressed Esophageal Squamous Carcinoma Growth by Blocking EGFR Activation and Inducing Cell Cycle Arrest

Figure 4

Isoliquiritigenin (ILQ) induced cell cycle arrested at the G0/G1 phase. (a) ILQ blocked the division of ESCC cells. KYSE-140 cell was treated with different ILQ for 24 h, and the expression of phosphor-histone H3 was detected by immunofluorescence. (b) ILQ induced G0/G1 cycle arrest. After treatment with ILQ for 24 h, the cells were stained with PI, and cell phased distribution was analyzed by FACS. (c) ILQ decreased the expression of cyclin D1. After the treatment of ILQ, the expression of indicated proteins was determined by western blotting.

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