A Simple RFLP-Based Method for HFE Gene Multiplex Amplification and Determination of Hereditary Hemochromatosis-Causing Mutation C282Y and H63D Variant with Highly Sensitive Determination of Contamination

Figure 2

Contamination tracking. Two different DNAs, one C282Y -/- and H63D +/- and one C282Y -/- and H63D -/-, were mixed at a 1 : 10 ratio. In (a), an extra FGA allele is present (long arrow) corresponding to one of the FGA alleles of the contaminating DNA. Moreover, an allele the size of the stutter (in base pair) is superimposed on the stutter peak. It is detected because the size (in RFU) is above the expected stutter size. (b) The arrow points to an extra SE33 allele from the contaminating DNA. (d) The arrow points to the contaminating H63D allele.