miR-520h Stimulates Drug Resistance to Paclitaxel by Targeting the OTUD3-PTEN Axis in Breast Cancer

<div>OTUD3 is targeted by miR-520h and associated with the miR-520h-mediated drug resistance. (a) Luciferase activity of OTUD3 3<svg height="11.4859pt" id="M17" style="vertical-align:-0.04979992pt" version="1.1" viewbox="-0.0498162 -11.4361 3.89517 11.4859" width="3.89517pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M310 541L304 571C290 586 211 619 185 610L80 76L131 52L310 541Z"></path></g></svg>UTR in MCF-7 cells transfected with miRNA-520h. (b) Effects of miR-520h overexpression on the expression of OTUD3 mRNA and protein. (c) Expression of OTUD3, PTEN, and p-AKT in MCF-7 breast cancer cells was detected by Western blot. (d) The OTUD3 and PTEN levels were determined by RT-PCR analysis after miR-520h overexpression. (e) The apoptosis of MCF-7/sh-OTUD3/Vec, MCF-7/sh-OTUD3/miR-520h treated with 5 nM of paclitaxel for 24 h and then assayed by flow cytometry analysis. (f) Overexpression of OTUD3 significantly inhibits miR-520h-induced MCF-7 cell viability compared to the control group. 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BioMed Research International

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Figure 3

Figure 3: miR-520h Stimulates Drug Resistance to Paclitaxel by Targeting the OTUD3-PTEN Axis in Breast Cancer 