lncRNA-TINCR Functions as a Competitive Endogenous RNA to Regulate the Migration of Mesenchymal Stem Cells by Sponging miR-761

Figure 1

TINCR promoted cell migration. lvTINCR and siTINCR were transfected into rMSCs, respectively. (a) Transwell assays. Cells with a density of cells per well were plated to the upper chamber of the Transwell chamber with 10% FBS containing the medium at the bottom layer. Incubated for 12 h at 37°C, the upper layer of the membrane was scraped and stained with 0.5% Crystal Violet Staining Solution and photographed under a microscope. (b) The number of migrated cells was quantified per well under a microscope by averaging five random fields (, ). (c) Wound healing assays. After transfection and scratch, the cells were photographed and counted under a phase contrast microscope 12 h later. Yellow and red lines showed start and end (12 h) positions of rMSCs after scraping. (d) Quantitative results of wound healing assays. The scratch area was observed under a phase contrast microscope and photographed (, ). All data are presented as .

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